The production of peptides is generally divided into two methods: chemical synthesis and biosynthesis.
1. Chemical synthesis: Chemical synthesis is a common polypeptide production method, which builds polypeptide chains by gradually connecting amino acid units through organic synthesis reactions. This method can produce peptides of various lengths and sequences. The steps of chemical synthesis include:
- Selection of appropriate protective bases and activation reagents;
- The amino acid units are connected step by step, and the reaction is carried out with appropriate activators and coupling reagents;
- Perform steps such as de-protection and purification to finally obtain the target polypeptide.
2. Biosynthesis: Biosynthesis is the use of biosynthetic mechanisms in organisms to produce peptides. One common approach is to use genetic engineering techniques to express target peptides in bacteria such as Escherichia coli or yeast. Steps include:
- Design the gene sequence of the target polypeptide and insert it into the appropriate expression vector;
- Transform bacteria or yeast cells and culture and induce expression;
- The expression products are harvested and purified polypeptides are obtained through purification and other processing steps.
Whether it is chemical synthesis or biosynthesis, the production of peptides requires strict control of reaction conditions, purification steps and quality testing to ensure the purity and quality of the peptides. Specific production methods and procedures are adjusted and optimized based on factors such as the length, sequence and use of the peptide. In practice, it is necessary to choose the appropriate method to produce polypeptides according to the specific situation.
